Kahn Tube Test with Dropping-pipettes

Dye loading with patch pipettes.

This protocol describes the loading of individual cells with fluorescent probes via patch pipettes. The patch-clamp methodology has been successfully used for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A broad range of dyes can be used with this loading technique. Markers for morphological reconstruction (e.g., Lucifer yellow); ion-sensitive indicator ...

A Drop Pipette for Use in the Kahn Test

Test tube systems with cutting/recombination operations.

We introduce test tube systems based on operations that are closely related to the splicing operation, i.e. we consider the operations of cutting a string at a specific site into two pieces with marking them at the cut ends and of recombining two strings with specifically marked endings. Whereas in the splicing of two strings these strings are cut at specific sites and the cut pieces are recomb...

Test-Tube Baby

A test-tube baby [4] is the product of a successful human reproduction that results from methods beyond sexual intercourse [5] between a man and a woman and instead utilizes medical intervention that manipulates both the egg [6] and sperm [7] cells for successful fertilization [8]. The term was originally used to refer to the babies born from the earliest applications of artificial insemination...

Cortactin in the Test Tube

stress fibers, do not contain cortactin. Despite the substantial size of the cortactin literature (currently the word “cortactin” appears in 339 articles), the most fundamental questions are still open. Thus, although cortactin has been implicated in the above-mentioned processes, its exact role is still not clearly defined. Its obvious participation in many of these fundamental events together...